TCA Cycle Interview Questions & Answers

Posted On:May 25, 2019, Posted By: Latest Interview Questions, Views: 6325, Rating :

Best TCA cycle Interview Questions and Answers

Dear Readers, Welcome to TCA cycle Objective Questions and Answers have been designed specially to get you acquainted with the nature of questions you may encounter during your Job interview for the subject of TCA cycle Multiple choice Questions. These Objective type TCA cycle Questions are very important for campus placement test and job interviews. As per my experience good interviewers hardly plan to ask any particular question during your Job interview and these model questions are asked in the online technical test and interview of many Medical Industry.

1. How many molecules of ATPs are synthesized per NADH oxidation?

A. 1

B. 2

C. 3

D. 4

Answer: C

Interview Questions On TCA Cycle

2. Why is the TCA cycle the central pathway of metabolism of the cell?

A. It occurs in the center of the cell

B. Its intermediates are commonly used by other metabolic reactions

C. All other metabolic pathways depend upon it

D. None of the above

Answer: B


3. In what form does the product of glycolysis enter the TCA cycle?

A. AcetylCoA

B. Pyruvate


D. Glucose

Answer: A


4. Malate-asparatate shuttle operates in

A. lungs and liver

B. heart and liver

C. pancreas and liver

D. none of these

Answer: B


5. Oxidation of a molecule involves

A. gain of electron

B. loss of electron

C. gain of proton

D. loss of proton

Answer: B


6. During cellular respiration, most of the ATP made, is generated by

A. oxidative phosphorylation

B. photophosphorylation

C. glycolysis

D. substrate-level phosphorylation

Answer: A


7. Important function of cholesterol is to

A. modulate fluidity

B. enhance blood circulation

C. prevent bile salts formation

D. None of these

Answer: B


8. To stop ATP synthesis which chemical is generally used?


B. 2,4 dinitrophenol


D. None of the chemical can stop ATP synthesis

Answer: B


9. The enzymes of the TCA cycle in a eukaryotic cell are located in the

A. nucleus

B. mitochondria

C. plasma membrane

D. lysosomal bodies

Answer: B


10. Citric acid cycle occurs in

A. cytoplasm

B. mitochondria

C. endoplasmic reticulum

D. golgi bodies

Answer: B


11. Most multi-cellular organisms obtain energy for the synthesis of ATP during oxidative phosphorylation from

A. high energy phosphate compounds

B. a proton gradient across the inner mitochondrial membrane

C. a proton gradient across the cell membrane

D. a proton gradient across the outer mitochondrial membrane

Answer: B


12. Standard redox potential for a substance is measured under standard condition and is expressed as

A. mili-Ampere

B. Volt

C. without unit

D. Ohm

Answer: B


13. Most of the enzymes of the citric acid cycle in a eukaryotic cell are located in the

A. inner mitochondrial membrane

B. cytosol

C. mitochondrial matrix

D. intermembrane space

Answer: C


14. A positive redox potential means substance has

A. lower affinity for electron

B. higher affinity for electron

C. lower affinity for proton

D. higher affinity for proton

Answer: B


15. The end product of glycolysis is pyruvate, which enters the citric acid cycle after being converted to

A. acetic acid

B. acetyl-CoA

C. acetaldehyde

D. none of these

Answer: B


16. Energy that is released from glucose during respiration but not transferred to ATP bonds can be detected as

A. CO2 



D. heat

Answer: D


17. Which of the following is involved in energy production?

A. Generation of proton gradients across membranes

B. Transport of electrons on organic molecules

C. Conversion of compounds with high energy to those of low energy

D. All of the above

Answer: D


18. Which of the following enzyme does not take part in the TCA cycle?

A. Citrate synthase

B. Iso-citrate dehydrogenase

C. Pyruvate dehydrogenase

D. Malate dehydrogenase

Answer: C


19. The first intermediate in TCA cycle is

A. succinate

B. fumerate

C. citrate

D. malate

Answer: C


20. Cholesterol can be synthesized de novo in

A. pancreas

B. intestine

C. liver

D. cell membrane

Answer: C


21. Which one of the following is not the intermediate of Kreb’s cycle?

A. Isocitrate

B. Succinate

C. Fumarate

D. Stearate

Answer: D


22. Which one is not the main protein in electron transport chain?

A. NADH dehydrogenase

B. Cytochrome bc1 complex

C. Cytochrome oxidase

D. Citrate synthease

Answer: D


23. How many ATPs are produced during citric acid cycle?

A. 10

B. 13

C. 12

D. 8

Answer: C


24. The FADH2 and NADH produced by the oxidation of one acetyl-CoA results in the synthesis of about-

A. 3 ATPs

B. 6 ATPs

C. 11 ATPs

D. 15 ATPs


Answer: C


25. In eukaryotes, electron transport occurs in

A. membranes and mitochondria

B. endoplasmic reticulum

C. cytoplasm

D. all of the above

Answer: A


26. The catabolism of sugars and fatty acids is similar because

A. both of these compounds are funnelled through the TC A/citric acid cycle

B. both of these compounds generate redox energy during catabolism

C. both of these compounds generate chemical energy during catabolism

D. all of the above

Answer: A