Microchemistry Multiple choice Questions & Answers

Best Microchemistry Objective type Questions and Answers

Dear Readers, Welcome to Microchemistry Objective Questions and Answers have been designed specially to get you acquainted with the nature of questions you may encounter during your Job interview for the subject of Microchemistry Multiple choice Questions. These Objective type Microchemistry Questions are very important for campus placement test and job interviews. As per my experience good interviewers hardly plan to ask any particular question during your Job interview and these model questions are asked in the online technical test and interview of many Medical Industry.

1.In electrophoresis, if the pH is above isoelectric point a protein will:

a) Precipitate

b) Not move        

c) Form a zwitter ion

d) Migrate to anode

e) Migrate to cathode

2.Regarding iso electric pH, true is (regarding enzymes)

a) All amino acids exit as zwitter ions     

b) Net + ve on molecule are equal

c) Enzyme activity maximum

d) None of the above   

 

3.Not produced in Adrenaline synthesis pathway

a) DOPA

b) Dopamine

c) Nor adrenaline

d)Tyramine   

 

4. 17- Alpha hydroxylase deficiency causes:

a) Decreased testosterone

b) Decrease of estrogens

c) Decrease glucagon

d) Increase estrogen   

 

5.  In ECF main electrolyte is

a) Na+

b) K+

c) Cl-

d)proteins   

 

6.Norepinephrine is converted to epinephrine by

a) Dopamine hydroxylase

b) Dopa decarboxylase

c) O-mefhylation

d) N-methylation   

 

7.Insulin promotes transport of glucose in which of these

a) Hepatocytes

b) Muscles         

c)RBC   

 

8. Regarding uncouple of Oxidative phosphorylation, the following statement is correct      

a) ATP production is more in anaerobic metabolism

b) Oxygen is not used and ATP is produced

c) Oxygen is used and ATP is not produced

d) Phosphorylation occurs in cytoplasm   

 

9.  Rate limiting step in adrenaline synthesis is conversion of:

a) Tyrosine to Dopa

b) Phenylalanine to tyrosine

c) Dopa to Dopamine

d) Dopamine to Norepinephrine   

 

10.Calcitonin is antagonist to

a) Parathyroid hormone

b) Thyroxine        

c) Triiodotyrosine   

d) Serotonin   

 

11.Which of the following is precursor of adrenalin and thyroxine synthesis

a) Phenyl alanine

b) Tyrosine

c) Tryptophan

d) None of the above   

 

12.Which of the following about biological membrane is true?

a) Lipid moiety is amphipathic              

b) Rigid assembly of protein, lipid and carbohydrate

c) Symmetrical bi leaflet structures        

d) Lipid and protein moiety are held by coyalent interactions   

 

13. To keep blood pHat 7.4 the HC03:H2C03 ratio should be

a) 20:1

b) 30:1

c) 15:1

d) 1:1   

 

14. Chemically, oestrogens are characterized by:

a) Absence of 02 at D-17

b) Aromatic character at D-ring

c) Absence of methyl group at C 10

d) A 21 - carbon steroid skeleton   

 

15.  Skeletal muscle is deficient in

a) Glucose - 6 - phosphatase 

b) Hexokinase

d) Phosphofructokinase

c) Isomerase   

 

16.  The most important buffer pair RBCs engaged in maintaining base equilibrium of blood is

a) Bicarbonate

b) Protein

c) Phosphate

d) Haemoglobin     

 

17.  Which of the following belongs to steroidal receptor super family

a) Vitamin D3

b)Encephalin

c)GHline

d) Phenylalanine   

 

18.The immediate precursor of norepinephrine is:

a) Epinephrine

b) Tyrosine

c) Dopai

d) Diphosphoglycerate 

e) ATP      

 

19.In mature erythrocytes the major available anion is:

a) Haemoglobin        

b) Chloride    

c) Bicarbonate

d)Bone   

 

20.Ferritin stores are present in following sites except

a) Intestine

b) Liver

c) Spleen

d) Adenyl cyclase   

 

21.Epinephrine activates the enzyme

a) Phosphodiesterase

b) Adenylate kinase

c)GH

d) Cortisone   

 

22.For which is cGMP second messenger

a) Thyroxine

b) Atrial natriuretic peptide   

 

23.  pH is maximum in:

a) Pancreatic secretion 

b) Intestinal secretions 

c) Salivary secretions

d) Gastric secretion   

 

24. S.G. of whole blood is:

a) 1.025-1.035

b) 1.035-1.045

c) 1.045-1.065

d) 1.065-1.075   

 

25.Which of the following amino acids is involved in the synthesis of thyroxine

a) Glycine

b) Methionine

c) Threonine

d) Tyrosine

 

26.Oxidative phosphorylation at the level of cytochrome oxidase is inhibited by

a) Carbon monoxide        

b) Cyanide

c) Rofenone

d) Oligomycin

 

27.  Hormone substrate concentration affects the velocity of enzymatic action, this is known as

a) Zimmerman reaction

b) Salkowski reaction

c) Michales Menton reaction

d) Lieberman-Burchard reaction

 

28.The most important buffer in extra cellular fluid is:

a) Hemoglobin

b) Protein

c) Phosphate

d)H203/HC02

 

29.Keratin in the nail is exceptionally strong than others because of

a) Disulphide bond   

b) Van der Wall's forces     

c) Ionic bonds

d) Calcification

 

30.   The normal specific gravity of urine is

a) 1.005-1.015

b) 1.015-1.020

c) 1.030-1.040 

d)None

 

31.The following statement about biological membrane is correct

a)They are symmetric bi leaflet structure

b)Their constituent lipid & protein moieties are held together by covalent teraction

c)They are rigid assemblies of protein, lipid & carbohydrates

d)Their lipid moieties are amphipathic in nature

 

32.Norepinephrine is converted to Epinephrine by

a) O-methylation

b) N-methylation

c) Dopamine hydroxylase       

d) Dopa decarboxylase

 

33.Disopropyl phosphofluoridate (DFP) reacts with serine proteases irreversibly and therefore is:

a) Allosteric inhibitor

b) Competitive inhibitor

c) Non competitive inhibitor

d) A repressor

 

34.Calcium as a second messenger in hormone action involves all except:

a) C-GMP                              

b) Na+ / Ca exchange pump

c)Protein kinase

d) Phosphatidyl inositol

 

   

 

35.The precursor of testosterone is:

a) Aldosterone                       

b) Methyl testosterone      

c) Estrone            

d) Pregnenolone

 

   

 

36.Insulin receptors are:

a) Tyrosine Kinase receptors

b) Phosphodiesterase        

c) Calcium-calmodulin

d)Lipoprotein lipase

e) All

 

   

 

37.Estrogen receptors are seen in

a) Microsomes                        

b) Membrane bound          

c) Nucleus           

d) Mitochondria

 

   

 

38.Insulin helps in all except

a) Glycogen synthesis                          

b) Gluconeogenesis           

c) Lipogenesis     

d) Glycolysis

 

   

 

39.All are second messengers, except:

a) c-AMP                 

b) Calcium                         

c)m-RNA                          

d) Phosphatidyl inositol

 

   

 

40.Exogenous catecholamines are metabolized by

a)COMT

b)MAO

c)MOPEG

d) All of the above

 

   

 

41.The major driving force for formation of membrane lipid bilayer is:

a) Hydrogen bonding     

b) Hydrophobic interactions       

c) Vander wall forces

d) Not known

 

   

 

42.Precursor of Testosterone is

a) Estrone                 

b) Cholesterol                    

c) Cortisol                         

d) Aldosterone

 

   

 

43.Which of the following amino acids is involved in the synthesis of thyroxine?

a) Glycine

b) Methionine

c) Threonine

d) Tyrosine

 

   

 

44.Which of the following is a synthetic reaction?

a) Reduction             

b) Acetylation                   

c) Oxidation                       

d) Hydrolysis

 

   

 

45.All are physiological buffers, except:

a) Proteins

b) Bicarbonates

c) Hb

d) CLPO,

 

   

 

46.Biosynthesis of oestrogens. Which is true:

a) Phenol end product                          

b) Aromatization occurs, which requires O, and NADPH

c) Angular methyl at C-10 is lost

d) Androgens serve as precursors

e) All of the above

 

   

 

47.True about Insulin is:

a) Is a glycoprotein

b) Has a half-life of 4 hours after intravenous injection

c)Increase protein synthesis from aminoacids in muscle

d)Increases the activity of glycogen phosphorylase

 

   

 

48.The most important buffer in RBC is

a) 02 Hb + Na Hemoglobinate

b) 0,Hb + K Hemoglobinate

c)H2C03 + KHC03

d)H2C03 + NaHC03

 

   

 

49.Regarding T3 and T4 which is incorrect?

a) T3 concentration is more                                 

b) T3 is more potent than T4

c) T3 and T4 have unequal biological activity      

d) Concentration of T4 is more than T3

 

   

 

50.Anterior pituitary gland secretes all except:

a)GRH                                   

b)GH                                

c)TSH                               

d)LH-FSH

 

   

 

51.Detoxification of drugs is controlled by:

a) Cytochrome

b) Cytochrome P450

c) Cytochrome C

d) Cytochrome A

 

   

 

52.Glucagon

a) Produces hypoglycaemia

b) Blocks release of catecholamines

c) Increases hyper peristalsis of intestine

d) Acts on heart

 

   

 

53.Which of the following is an effect of dinitrophenoi on oxidative phosphorylation

a) Inhibition of cytochrome b

b) Blockade of both electron transport and ATP synthesis

c)Inhibition of ATP synthesis with normal electron transport

d)Inhibition of electron transport but not ATP synthesis

 

   

 

54.Muscle cannot make use of glycogen for energy due to the absence of

a) Glucose-6-phosphotase                    

b) Glucokinase

c) Phosphorylase

d) Phosphoglucomutase

 

   

 

55.Insulin has amino acids

a)49

b)50

c)51

d)52

e)62

 

   

 

 

56.Which of the following hormone does not have a second messenger?

a) Insulin

b) Growth hormone

c) Aldosterone    

d) Somatostatin

 

   

 

57.All are true of absorption of calcium from the gut except:

a) Vitamin D increases the absorption

b) Parathormone increase it

c) Calcitonin decreases it

d) An alkaline pH in the gut decreases calcium absorption

 

   

 

58.Which of the following is not a second messenger?

a) cAMP

b) cGMP

c) Phosphatidyl inositol     

d) Phosphatidyl glycerol

 

   

 

59.The lipid bilayer of cell membrane exists as

a) Solid                     

b) Semisolid                       

c)Gel                                 

d) Fibers

 

   

 

60.Spontaneous chemical reaction means free energy change is

a) Positive

b) Negative

c) Zero

d) Infinity

 

   

 

61.Lamin is present in

a) Basement membrane

b) Inner nuclear membrane 

c) Outer nuclear membrane

d) Mitochondria

 

   

 

62.Which of these contain 19 carbon atoms:

a) Estradiol

b) Testosterone

c) Aldosterone

d) Cortisol

 

 

   

 

63.Which of the following is a direct precursor of hormones

a) Ergosterol

b) Cyclic AMP

c) Cholesterol

d) Tyrosine

 

   

 

64.In cytochrome p 450, P stands for

a) Structural proteins

b) Substrate protein

c) Pigment

d) Polymer

 

   

 

65.A substance having affinity but no intrinsic activity is

a) Agonist

b) Partial agonist

c) Antagonist

d) Physiological antagonist

 

   

 

66.   Osmolality is

a) Osmalarity per kg of solvent  

b) Osmalarity per liter of solvent 

c) Moles per kg of solvent

d) Moles per liter of solvent

 

   

 

67.Thyroxine and catecholamine are .derived from

a) Tyrosine

b) Tryptophan

c) Alanine

d) Leucine

 

   

 

68.Biological cell membrane contains all the following except

a) Steroid receptors   

b) Prostaglandins

c) Cholesterol

d) Cytochrome

 

   

 

69.The blood concentration of H2C03 (meq/L) is:

a) 5

b)15

c)20

d)50

 

   

 

70.What is known as the "second messenger"?

a) ATP

b)ADP

c) Cyclic AMP

d)GMP

 

   

 

71.  A bilipid layer is most permeable to:

a) Potassium

b) Sodium

c) Glucose 

d)Urea

 

   

 

 

 

72.A substance having affinity and intrinsic activity is:

a) Agonists

b) Partial agonist

c) Antagonist

d) Physiological antagonist

 

   

 

73.Which of the following hormones are dependent on cyclic AMP?

a) Vasopressin

b) Aldosterone

c) Renin

d) Angiotensin

 

   

 

74.Physiologically, this hormone is superfluous in adult humans

a) Calcitonin

b) Somatotropin

c) Prolactin

d)Somatostatin

 

   

 

75.  Which is correct about intracellular fluid

a) Amount less than ECF 

b) Amount more than ECF 

c) Amount equal to ECF

d) 70% of total body weight

 

   

 

76.Normal blood pH is

a)6.8-7.0

b)7.0-7.12

c)7.38-7.4

d) 7.7-8.0 

 

   

 

77.Km decreases but Vmax same-enzyme is

a) Competitive

b) Non-competitive

c) Irreversible

d) Uncompetitive

 

   

 

78.In health the pH of the blood lies between th£ range of

a) 6.5-7.1

b) 7.2-7.3

c) 7.35-7.45

d) 7.45-7.6

 

   

 

79.Hormone that mediates enzymatic action at cellular level is

a) Thyroxine

b) Insulin

c) Cortisol

d) vit-D

 

   

 

80.Multienzyme complex in humans

a) Fatty acid synthetase

b) Malonyl CoA Carboxylase

c) Carbamoyl phosphate synthetase

d) Adenosine phospho ribosyl transferase

 

   

 

81.Fidelity enzyme in protein synthesis

a) DNA polymerase

b) RNA polymerase

c) Amino-Acyl tRNA synthase

d) Amino acyl reductase

 

   

 

82.The enzyme acetylcholine esterase is synthesized in:

a) Liver

b) Plasma

c) Marrow

d) Spleen

e) Ferroprotoporphyrin

 

   

 

83.Dehydrogenases used as coenzymes all of the following, except:

a)FMN

b)FAD

c)NADP

d)NAD

 

   

 

84.Which inhibits adenyl cyclase enzyme

a) Somatostatin        

b) Calcitonin

c) Epinephrine

d)Thyroxine

 

   

 

85.Zinc is an essential component of the following enzymes except

a) Carbonic anhydrase

b) Alkaline phosphatase

c) Xanthine oxidase

d) Ethanol dehydrogenase

e)Carboxypeptidase

 

   

 

86.Which of the following enzymes do not need cofactor:

a) Oxidoreductase

b) Isomerase

c) Aldolase

d) Lyases

 

   

 

87. Enzyme that hydrolyzes sucrose is:

a) Invertase     

b) Amylase     

c) Emulsin

d) Sucrose phosphorylase

e) Saccharase

 

   

 

88.Tyrosinase is

a) Oxidase

b) Tranferase

c) Lyase

d) Sucrose phosphorylase

 

   

 

 

89.Coenzyme, in an enzymatic reaction usually functions to:

a) Activate the substrate

b) Increase the active sites of apoenzyme

c) Enhance the specificity of apoenzyme

d) Accept one of the cleavage products

 

   

 

 

90.Lactate dehydrogenase is:

a) Isozyme

b) Coenzyme

c) Antienzyme

d) Zymogen

 

   

 

91. Benzidine test is positive with the enzyme

a) Cytochrome oxidase

b) Dehydrogenase

c) Hydrolase        

d) Peroxidase

 

   

 

92.  Which of the following is not decreased in diabetes mellitus

a) Glucokinase

b) Acetyl CoA carboxylase

c) Pyruvate carboxylase     

d) All

 

   

 

93.Aldolase is an enzyme whose substrate is*

a) Glucose -6 phosphate        

b) Fructose-6-phosphate   

c) Fructose

d)Fructose- 1,6-biphosphate

 

   

 

94.Insulin stimulates the activity of the following enzymes except:

a) Acetyl CoA carboxylase

b) Pyruvate kinase

c) Hormone sensitive lipase

d) Glycerol kinase

e) Phosphofructokinase

 

   

 

95.  True statement regarding Enzyme cascade system is

a) Once activated, continues indefinitely 

b) Counter regulation is seen in all 

c) Activated by hormones

d) Substrate amplification occurs

 

   

 

96. Coenzymes are-organic compounds:

a) Lipoprotein

b) Proteinaceous

c) Non-protein

d) Any of the above

 

   

 

97. Enzyme deficiency in alkaptonuria is

a) Tyrosine hydroxylase 

b) Homogentisic acid oxidase 

c) Cystathionine synthase

d) Phenyl alanine hydroxylase

 

   

 

98.Michaelis-Menten hypothesis states that:

a)Rate of enzymatic reaction is independent of substrate concentration

b)Rate of nonenzymatic reaction is proportional to substrate concentration

c)Km is the enzyme-substrate complex association constant

d)Enzyme-substrate complex formation is essential in enzymatic reaction

 

   

 

99.Enzymes not important in testosterone biosynthesis:

a) 5-alpha reductase

b) Delta 5-4 isomerase

c) 18 hydroxylase

d) Alpha hydroxylase

 

   

 

100.When the prosthetic group of an enzyme is an inorganic ion, it is called a

a) Cofactor

b) Coenzyme

c) Apoenzyme

d) Holoenzyme  

 

101. Enzymes stored in muscle is:

a) Alkaline phosphatase

b)SGOT

c)SGPT

d)CPK