Nucleic Acids Interview Questions & Answers
Best Nucleic Acids Interview Questions and Answers
Dear Readers, Welcome to Nucleic Acids Objective Questions and Answers have been designed specially to get you acquainted with the nature of questions you may encounter during your Job interview for the subject of Nucleic Acids Multiple choice Questions. These Objective type Nucleic Acids Questions are very important for campus placement test and job interviews. As per my experience good interviewers hardly plan to ask any particular question during your Job interview and these model questions are asked in the online technical test and interview of many Medical Industry.
1. Because DNA is a highly charged polyanion, its stability to heat denaturation
A. does not depend on hydrophobic interactions
B. increases with increasing salt
C. is independent of G – C content
D. decreases with increasing salt
Answer: B
2. The sugar in RNA is __________ , the sugar in DNA is __________ .
A. deoxyribose, ribose
B. ribose, deoxyribose
C. ribose, phosphate
D. ribose, uracil
Answer: B
3. Nucleoside is a pyrimidine or purine base
A. covalently bonded to a sugar
B. ionically bonded to a sugar
C. hydrogen bonded to a sugar
D. none of the above
Answer: A
4. In gel electrophoresis, what fragments will move most quickly through a gel?
A. Large fragments
B. Small fragments
C. Large genome
D. None of these
Answer: B
5. Which pyrimidine base contains an amino group at carbon 4?
A. Cytosine
B. Thymine
C. Uracil
D. Adenine
Answer: A
6. Nucleotide bases and aromatic amino acids absorb light respectively at
A. 280 and 260 nm
B. 260 and 280 nm
C. 270 and 280 nm
D. 260 and 270 nm
Answer: B
7. The glycosidic bonds in DNA and RNA
A. connect the sugar to the base
B. can be hydrolyzed by OH- ion
C. stabilize Watson-Crick H-bonds
D. are free to rotate over about 180°
Answer: A
8. Nucleic acids can be analyzed experimentally by their
A. molecular weight
B. absorption of visible light
C. absorption of uv light
D. none of these
Answer: C
9. Thymidine
A. can participate in hydrophobic interactions due to its methyl group
B. is replaced by uracil in RNA
C. normally forms two hydrogen bonds with adenosine
D. all of the above
Answer: D
10. A nucleotide consists of
A. a sugar, a base and a phosphate
B. a sugar and a phosphate
C. paired bases
D. a sugar, a base and three phosphates
Answer: A
11. A five carbon sugar lacking a hydrogen at the number 2 carbon is found in
A. DNA
B. RNA
C. mRNA
D. rDNA
Answer: A
12. The unfavourable free energy associated with not satisfying a hydrogen bond after formation of the double helix is approximately
A. 10 kJ/mol
B. 20 kJ/mol
C. 25 kJ/mol
D. 30 kJ/mol
Answer: B
13. The most stabilizing force for nucleic acids is
A. hydrogen bonds
B. electrostatic bond
C. Van der Waals
D. conformational entropy
Answer: C
14. A purine with an amine (NH2) group on the 6th carbon is
A. adenine
B. cytosine
C. thymine
D. guanine
Answer: A
15. Which of the following is found on RNA but not DNA?
A. Uracil
B. Deoxyribose
C. Phosphate
D. Adenine
Answer: A